A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Stem cell transplantation in poor-risk chronic lymphocytic leukemia: assessment of post-transplant minimal residual disease using four- and six-color flow cytometry and allele-specific RQ-PCR
Tekijät: Itälä, Maija; Huhtinen, Anna-Riina; Juvonen, Vesa; Kairisto, Veli; Pelliniemi, Tarja-Terttu; Penttilä, Tarja-Leena; Rauhala, Auvo; Tienhaara, Anri; Remes, Kari
Kustantaja: WILEY-BLACKWELL
Kustannuspaikka: MALDEN
Julkaisuvuosi: 2008
Journal: European Journal of Haematology
Tietokannassa oleva lehden nimi: EUROPEAN JOURNAL OF HAEMATOLOGY
Lehden akronyymi: EUR J HAEMATOL
Vuosikerta: 81
Numero: 2
Aloitussivu: 100
Lopetussivu: 106
Sivujen määrä: 7
ISSN: 0902-4441
DOI: https://doi.org/10.1111/j.1600-0609.2008.01082.x
Verkko-osoite: https://doi.org/10.1111/j.1600-0609.2008.01082.x
A total of 178 bone marrow samples were taken for minimal residual disease (MRD) analysis after 34 stem cell transplantations for poor-risk chronic lymphocytic leukemia, and 86 of them were analyzed in parallel by flow cytometry and allele-specific oligonucleotide-PCR (ASO-PCR). ASO primer was successfully designed for all patients whose frozen diagnosis samples were available. Flow cytometry and ASO-PCR were concordant, i.e. both either positive or both negative, in 78% of the analyses. Flow cytometry did not detect MRD in any of the samples that were PCR-negative cases. In contrast, ASO-PCR detected MRD in samples that were negative for MRD by flow cytometry in 22% of the analyses. In one patient, the immunophenotype but not the IgV(H) gene sequence had changed during a course of the disease, and MRD could not be followed by flow cytometry. In the remaining cases, the discrepancy was due to a higher sensitivity of ASO-PCR. Autologous stem cell transplantation resulted in clinical complete response in 87% (20/23) of the patients. By flow cytometry, 35% (8/23) of autotransplanted patients became MRD-negative, but only 12.5% (2/16) PCR-negative (sensitivity of ASO-PCR < 0.001 and < 0.01, respectively). All allotransplanted patients achieved or maintained hematological CR, and five out of nine patients (56%) became PCR-negative (sensitivity of PCR between < 0.001 and < 0.003), two of them having non-myeloablative conditioning. None of the patients who became PCR-negative after allogeneic transplantation have relapsed.
