ECOCRIN project adopts the ecosystem perspective on cultural and creative sectors. We defined an ecosystem as a community of interdependent stakeholders that create and enable the social context and networks supporting cultural and creative individuals' and companies' activities (e.g., Isenberg, 2010; Stam, 2015). We aimed to build a comprehensive ecosystem mapping, which identifies the key stakeholders that support sustainability and emergence of cultural and creative sectors. Instead of categorizing cultural and creative sectors' actors as outputs of ecosystem, we included them in the analysis to identify their needs. This choice differs from concurrent ecosystem approaches where the focus is solely on stakeholders who generate and maintain ecosystems (Stam & van de Ven, 2021). Moreover, our mapping covers both cultural and creative industries even if they differ among others in their not-for-profit and for-profit orientations (De Bernard et al., 2022). First, we examined local creative ecosystems through desk research by contextual and geographical mapping of key stakeholders and their inputs in the empirical case regions of ECOCRIN project, Turku and Paimio, Pori, and Kuhmo. Second, we conducted personal interviews (n = 26) with individuals and companies in each case location across different cultural and creative sectors. Finally, we conducted an international benchmark on regional creative ecosystems of Lund from Sweden and Liepãja from Latvia.