A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Redesign of the Chlamydomonas reinhardtii QB binding niche reveals photosynthesis works in the absence of a driving force for QA‐QB electron transfer




TekijätLambreva, Maya D.; Zobnina, Veranika; Antal, Taras K.; Peeva, Violeta N.; Giardi, Maria Teresa; Bertalan, Ivo; Johanningmeier, Udo; Virtanen, Olli; Ray, Mithila; Mulo, Paula; Polticelli, Fabio; Tyystjärvi, Esa; Rea, Giuseppina

KustantajaWiley

Julkaisuvuosi2024

JournalPhysiologia Plantarum

Tietokannassa oleva lehden nimiPhysiologia Plantarum

Artikkelin numeroe70008

Vuosikerta176

Numero6

ISSN0031-9317

eISSN1399-3054

DOIhttps://doi.org/10.1111/ppl.70008

Verkko-osoitehttps://doi.org/10.1111/ppl.70008

Rinnakkaistallenteen osoitehttps://research.utu.fi/converis/portal/detail/Publication/478046305


Tiivistelmä

An in silico redesign of the secondary quinone electron acceptor (QB) binding pocket of the D1 protein of Photosystem II (PSII) suggested that mutations of the F265 residue would affect atrazine binding. Chlamydomonas reinhardtii mutants F265T and F265S were produced to obtain atrazine-hypersensitive strains for biosensor applications, and the mutants were indeed found to be more atrazine-sensitive than the reference strain IL. Fluorescence and thermoluminescence data agree with a weak driving force and confirm slow electron transfer but cannot exclude an additional effect on protonation of the secondary quinone. Both mutants grow autotrophically, indicating that PSII requires strong light for optimal function, as was the case in the ancestral homodimeric reaction center.


Ladattava julkaisu

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Julkaisussa olevat rahoitustiedot
Regione Lazio, Grant n. 85-2017-15256; European Cooperation in Science and Technology, COST Action TD1102; Novo Nordisk Fonden, grant NNF220C0079284, Academy of Finland, grant 333421, CNR project FOE-2021, grant dba.ad005.225


Last updated on 2025-10-02 at 14:11