A1 Refereed original research article in a scientific journal
Pandemic influenza A(H1N1 pdm09) vaccine induced high levels of influenza-specific IgG and IgM antibodies as analyzed by enzyme immunoassay and dual-mode multiplex microarray immunoassay methods
Authors: Kazakova A, Kakkola L, Ziegler T, Syrjänen R, Päkkilä H, Waris M, Soukka T, Julkunen I
Publisher: ELSEVIER SCI LTD
Publication year: 2020
Journal: Vaccine
Journal name in source: VACCINE
Journal acronym: VACCINE
Volume: 38
Issue: 8
First page : 1933
Last page: 1942
Number of pages: 10
ISSN: 0264-410X
eISSN: 1873-2518
DOI: https://doi.org/10.1016/j.vaccine.2020.01.022
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/46507504
Influenza A viruses continue to circulate throughout the world as yearly epidemics or occasional pandemics. Influenza infections can be prevented by seasonal multivalent or monovalent pandemic vaccines. In the present study, we describe a novel multiplex microarray immunoassay (MAIA) for simultaneous measurement of virus-specific IgG and IgM antibodies using Pandemrix-vaccinated adult sera collected at day 0 and 28 and 180 days after vaccination as the study material. MAIA showed excellent correlation with a conventional enzyme immunoassay (EIA) in both IgG and IgM anti-influenza A antibodies and good correlation with hemagglutination inhibition (HI) test. Pandemrix vaccine induced 5-30 fold increases in anti-H1N1pdm09 influenza antibodies as measured by HI, EIA or MAIA. A clear increase in virus-specific IgG antibodies was found in 93-97% of vaccinees by MAIA and EIA. Virus-specific IgM antibodies were found in 90-92% of vaccinees by MAIA and EIA, respectively and IgM antibodies persisted for up to 6 months after vaccination in 55-62% of the vaccinees. Pandemic influenza vaccine induced strong anti-influenza A IgG and IgM responses that persisted several months after vaccination. MAIA was demonstrated to be an excellent method for simultaneous measurement of antiviral IgG and IgM antibodies against multiple virus antigens. Thus the method is well suitable for large scale epidemiological and vaccine immunity studies. (C) 2020 Elsevier Ltd. All rights reserved.
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