A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Glutaraldehyde-enhanced autofluorescence as a general tool for 3D morphological imaging
Tekijät: Niemeläinen, Miika; Haapanen-Saaristo, Anna-Mari; Koskinen, Leena M.; Gullmets, Josef; Peuhu, Emilia; Meinander, Annika; Calhim, Sara; Paatero, Ilkka
Kustantaja: The Company of Biologists
Julkaisuvuosi: 2024
Journal: Biology Open
Tietokannassa oleva lehden nimi: Biology Open
Lehden akronyymi: Biol Open
Artikkelin numero: bio060428
Vuosikerta: 13
Numero: 11
ISSN: 2046-6390
eISSN: 2046-6390
DOI: https://doi.org/10.1242/bio.060428
Verkko-osoite: http://doi.org/10.1242/bio.060428
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/458885894
Routine histochemical techniques are capable of producing vast amount of information from diverse samples types, but these techniques are limited in their ability to generate 3D information. Autofluorescence imaging can be used to analyse samples in 3D but it suffers from weak/low signal intensities. Here, we describe a simple chemical treatment with glutaraldehyde to enhance autofluorescence for 3D fluorescence imaging and to generate detailed morphological images on whole-mount samples. This methodology is straightforward and cost-effective to implement, suitable for a wide range of organisms and sample types. Furthermore, it can be readily integrated with standard confocal and fluorescence microscopes for analysis. This approach has the potential to facilitate the analysis of biological 3D structures and research in developmental biology, including studies on model and non-model organisms.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Julkaisussa olevat rahoitustiedot:
Business Finland (1718/31/2021, I.P.), FinnFOTO (20220515, I.P.), the Åbo Akademi University Foundation (AM), Sigrid Jusélius Foundation (E.P), Academy of Finland research fellowship (323096, E.P.), the Hospital District of Southwest Finland (11083, E.P.), Turku Doctoral Program of Molecular Medicine (L.M.K.) Open Access funding provided by University of Turku: Turun Yliopisto. Deposited in PMC for immediate release.
