Detection of Tn-antigen in breast and prostate cancer models by VVL-labeled red dye-doped nanoparticles - UTU Research Portal

A1 Refereed original research article in a scientific journal

Detection of Tn-antigen in breast and prostate cancer models by VVL-labeled red dye-doped nanoparticles

Authors: Verhassel, Alejandra; Kimani, Martha; Gidwani, Kamlesh; Sandholm, Jouko; Gawlitza, Kornelia; Rurack, Knut; Härkönen, Pirkko

Publisher: Taylor and Francis Ltd.

Publication year: 2024

Journal: Nanomedicine

Journal name in source: Nanomedicine

Volume: 19

Issue: 29

First page : 2463

Last page: 2478

ISSN: 1743-5889

eISSN: 1748-6963

DOI: https://doi.org/10.1080/17435889.2024.2405454

Web address : https://doi.org/10.1080/17435889.2024.2405454

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/458628849

Abstract

Aim: Fluorescence detection of breast and prostate cancer cells expressing Tn-antigen, a tumor marker, with Vicia villosa lectin (VVL)-labeled nanoparticles.

Materials & methods: Breast and prostate cancer cells engineered to express high levels of Tn-antigen and non-engineered controls were incubated with VVL-labeled or unlabeled red dye-doped silica-coated polystyrene nanoparticles. The binding to cells was studied with flow cytometry, confocal microscopy, and electron microscopy.

Results: Flow cytometry showed that the binding of VVL-labeled nanoparticles was significantly higher to Tn-antigen-expressing cancer cells than controls. Confocal microscopy demonstrated that particles bound to the cell surface. According to the correlative light and electron microscopy the particles bound mostly as aggregates.

Conclusion: VVL-labeled nanoparticles could provide a new tool for the detection of Tn-antigen-expressing breast and prostate cancer cells.

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Funding information in the publication:
The study was funded as part of the European Network ‘GlycoImaging’ by the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Action grant agreement No. 721297. Working grants were received from the University of Turku Graduate School (Drug Research Doctoral Programme), Ida Montinin Säätiö (The Ida Montin Foundation), K. Albin Johansson Foundation, Paulo Foundation and Lounais-Suomen Syöpäyhdistys (The Cancer Society of Southwestern Finland).