A1 Refereed original research article in a scientific journal
Detection of anti-enterovirus IgG in human sera by ELISA method using the KTL-510 peptide
Authors: Pellerova, Michaela; Albertova, Katarina; Simkova, Vanesa; Borsanyiova, Maria; Benkoova, Brigita; Kissova, Renata; Pastuchova, Katarina; Tauriainen, Sisko; Galama, Jochem M. D.; Bopegamage, Shubhada
Publisher: FRONTIERS MEDIA SA
Publishing place: LAUSANNE
Publication year: 2024
Journal: Acta Virologica
Journal name in source: ACTA VIROLOGICA
Journal acronym: ACTA VIROL
Article number: 12739
Volume: 68
Number of pages: 9
ISSN: 0001-723X
eISSN: 1336-2305
DOI: https://doi.org/10.3389/av.2024.12739
Web address : https://doi.org/10.3389/av.2024.12739
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/458625584
Enterovirus (EV) infections occur frequently in humans. In some geographical areas they are more common. These viruses cause diseases with varying degrees of severity, from a simple respiratory tract infection to severe diseases. Since EVs include more than 70 serotypes currently circulating in the population, a methodology that detects most of them is needed. ELISA is a rapid, sensitive, and economical diagnostic method for the identification of EV serotypes and can also be used as a retrospective diagnostic tool or in the investigation of outbreaks of infection. Commercial EV-ELISAs often appear and gradually disappear from the market supply. We have used the KTL-510 peptide, a synthetic viral protein of poliovirus VP1, as an antigen in a peptide-based ELISA for the detection of a broader spectrum of anti-EV antibodies. We aimed to design, optimize, and standardize this in-house ELISA with the peptide, and implement the method for routine detection of anti-EV IgG in human sera. For determining the cut-off value, we used 100 patients' sera which were previously tested negative for IgG antibodies against EVs using a commercial ELISA kit available. We monitored patients' sera samples sent for serological testing of anti-coxsackievirus antibodies to the National Reference Center for the Identification of Enteric Viruses between 2018-2022. These serum samples were examined using a standard virus neutralization test as well as the newly developed ELISA method.
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Funding information in the publication:
The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by National Reference Center for Identification of Enteric Viruses and Slovak Medical University internal grant number 05/2021-SVG1.
