Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards
: Torta F., Hoffmann N., Burla B., Alecu I., Arita M., Bamba T., Bennett S.A.L., Bertrand-Michel J., Brügger B., Cala M.P., Camacho-Muñoz D., Checa A., Chen M., Chocholoušková M., Cinel M., Chu-Van E., Colsch B., Coman C., Connell L., Sousa B.C., Dickens A.M., Fedorova M., Eiríksson F.F., Gallart-Ayala H., Ghorasaini M., Giera M., Guan X.L., Haid M., Hankemeier T., Harms A., Höring M., Holčapek M., Hornemann T., Hu C., Hülsmeier A.J., Huynh K., Jones C.M., Ivanisevic J., Izumi Y., Köfeler H.C., Lam S.M., Lange M., Lee J.C., Liebisch G., Lippa K., Lopez-Clavijo A.F., Manzi M., Martinefski M.R., Math R.G.H., Mayor S., Meikle P.J., Monge M.E., Moon M.H., Muralidharan S., Nicolaou A., Nguyen-Tran T., O'Donnell V.B., Orešič M., Ramanathan A., Riols F., Saigusa D., Schock T.B., Schwartz-Zimmermann H., Shui G., Singh M., Takahashi M., Thorsteinsdóttir M., Tomiyasu N., Tournadre A., Tsugawa H., Tyrrell V.J., van der Gugten G., Wakelam M.O., Wheelock C.E., Wolrab D., Xu G., Xu T., Bowden J.A., Ekroos K., Ahrends R., Wen
Publisher: Nature Portfolio
: 2024
: Nature Communications
: Nature communications
: 8562
: 15
: 2041-1723
DOI: https://doi.org/10.1038/s41467-024-52087-x(external)
: https://doi.org/10.1038/s41467-024-52087-x(external)
: https://research.utu.fi/converis/portal/detail/Publication/458528172(external)
In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
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1,2 Work in the MRW laboratory is supported by grants from the National University of Singapore via the Life Sciences Institute (LSI) and A*STAR IAF-ICP I1901E0040. 4 N.H. acknowledges support by de.NBI and the de.NBI Cloud within the German Network for Bioinformatics Infrastructure (de.NBI) and ELIXIR-DE (Forschungszentrum Jülich and W-de.NBI-001, W-de.NBI-004, W-de.NBI-008, W-de.NBI-010, W-de.NBI-013, W-de.NBI-014, W-de.NBI-016, W-de.NBI-022). 5 Bundesministerium für Bildung, Wissenschaft und Forschung” BMBFW within the project DigiOmics4Austria. 6 Work in the SALB laboratory is supported by grants from the Canadian Institutes of Health Research and the Canadian Consortium on Neurodegeneration and Aging CAN-CIHR 163902 and PJT 183604. 7,8,55,56 H.T. was supported by JSPS KAKENHI (21K18216 to Hiroshi T.), the National Cancer Centre Research and Development Fund (2020-A-9, H.T.), and JST ERATO Grant (JPMJER2101 to H.T.). 9 This research is partly supported by AMED-BINDS (JP23ama121055), JST A-STEP (JPMJTR204J), JST-NBDC (JPMJND2305), and the MEXT Cooperative Research Project Program, Medical Research Center Initiative for High Depth Omics, and CURE:JPMXP1323015486 for the Medical Institute of Bioregulation, Kyushu University. 10 Work in MetaToul (Toulouse metabolomics & fluxomics facilities, www.mth-metatoul.com) part of the French National Infrastructure for Metabolomics and Fluxomics is supported by MetaboHUB-ANR-11-INBS-0010 and Inserm (French National Institut for Research in Health). 13 Work at the University of Manchester supported in part by grants from the British Heart Foundation (PG/2019/34923) and BBSRC (BB/W006022/1). 16 Work at University of Pardubice was supported by grant No. 21-20238S (Czech Science Foundation) and ERC Adv grant No. 101095860 (European Research Council). 19 Work in the MTS department is supported by the MetaboHUB infrastructure (ANR-11-INBS-0010 grant). 21 BBSRC for funding to the Babraham Institute and to Michael Wakelam’s memory for his life of contribution to the lipidomics world. 23 Mass spectrometeric analysis was performed at the Turku Metabolomics Centre with the support of Biocenter Finland. 24,25,26 This publication is based upon work from COST Action EpiLipidNET, Pan-European Network in Lipidomics and Epilipidomics (CA19105; https://www.epilipid.net), supported by COST (European Cooperation in Science and Technology).” 30 M.G. is an early-stage researcher supported by the H2020 ITN consortium ArthritisHeal (#812890). M.G. was partially supported by the NWO XOmics project #184.034.019. 31 Nanyang Assistant Professorship, Lee Kong Chian School of Medicine, Nanyang Technological University. 36 Supported by grants from the National Natural Science Foundation of China (21934006 and 22074144). 48 Ser Cymru Project Sepsis grant funded by Welsh Government/EU-ERDF (VJT, VBO). 51,52 Supported by the Tohoku Medical Megabank Project from MEXT, the Japan Agency for Medical Research and Development (AMED; under grant numbers JP20km0105001 and JP21tm0124005) and KAKENHI Grant Number JP23H02625 [D.S.]. 54 The Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock has been funded by the Austrian Federal Ministry for Digital and Economic Affairs, the Austrian National Foundation for Research, Technology and Development as well as BIOMIN Holding GmbH, which is part of DSM. The authors also thank Marco Reiter for technical support. The participants would like to acknowledge Clay Davis from the National Institute of Standards and Technology. B.B. would like to acknowledge Christian Luechtenborg for his contribution to this project.
