A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Assessment of twelve echovirus virus-neutralisation assays in Europe: recommendations for harmonisation of non-polio enterovirus sero-surveillance studies
Tekijät: Couderé, Karen; Benschop, Kimberley S.M.; Koen, Gerrit; van Eijk Hetty; Harvala, Heli; Bailly, Jean-Luc; Bessaud, Maël; Kamau, Everlyn; Simon, Isabelle; Joffret, Marie-Line; Nikolaeva-Glomb, Lubomira; Georgieva, Irina; Stoyanova, Asya; Diedrich, Sabine; Böttcher, Sindy; Cabrerizo, Maria; Tabain, Irena; Hruškar, Željka; Stevanović, Vladimir; Susi, Petri; Hietanen, Eero; Palminha, Paula; Rainetová, Petra; Baicus, Anda; Kristensen, Maartje; Pajkrt, Dasja; Murk, Jean-Luc; Wolthers, Katja C.; and European non-polio enterovirus network
Kustantaja: Microbiology Society
Julkaisuvuosi: 2024
Journal: Journal of General Virology
Tietokannassa oleva lehden nimi: Journal of General Virology
Vuosikerta: 105
Numero: 9
ISSN: 0022-1317
eISSN: 1465-2099
DOI: https://doi.org/10.1099/jgv.0.001986
Verkko-osoite: https://doi.org/10.1099/jgv.0.001986
Non-polio enteroviruses (NPEV) cause significant disease worldwide. Population-based sero-surveillance, by measuring antibodies against specific NPEV types, provides additional information on past circulation and the prediction for future upsurges. Virus neutralisation assays (VNA), the current method of choice for measuring NPEV type specific antibodies, are not entirely standardised. Via the European Non-Polio Enterovirus Network, we organised a VNA quality assessment in which twelve laboratories participated. We provided five echovirus (E) types (E1, E18, E30 G2, E30 G6 and E6) and intravenous immunoglobulins (IVIG) as a sample for the NPEV VNA quality assessment. Differences in VNA protocols and neutralising Ab (nAb) titres were found between the participating laboratories with geometric coefficients of variation ranging from 10.3-62.9 %. Mixed-effects regression analysis indicated a small but significant effect of type of cell line used. Harmonisation of cell line passage number, however, did not improve variation between laboratories. Calibration by making use of a reference sample, reduced variation between laboratories but differences in nAb titres remained higher than two log2 dilution steps. In conclusion, sero-surveillance data from different laboratories should be compared with caution and standardised protocols are needed.
Julkaisussa olevat rahoitustiedot:
This study was supported by the: Centrum Infectieziektebestrijding, Rijksinstituut voor Volksgezondheid en Milieu Principle Award Recipient: Kimberley S.M. Benschop Instituto de Salud Carlos III (Award PI18CIII-00017) Principle Award Recipient: MariaCabrerizo
