A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Comparative proteomics analysis of the mouse mini-gut organoid: insights into markers of gluten challenge from celiac disease intestinal biopsies
Tekijät: Moulder, Robert; Bhosale, Santosh D.; Viiri, Keijo; Lahesmaa, Riitta
Kustantaja: FRONTIERS MEDIA SA
Kustannuspaikka: LAUSANNE
Julkaisuvuosi: 2024
Journal: Frontiers in Molecular Biosciences
Tietokannassa oleva lehden nimi: FRONTIERS IN MOLECULAR BIOSCIENCES
Lehden akronyymi: FRONT MOL BIOSCI
Artikkelin numero: 1446822
Vuosikerta: 11
Sivujen määrä: 15
eISSN: 2296-889X
DOI: https://doi.org/10.3389/fmolb.2024.1446822
Verkko-osoite: https://doi.org/10.3389/fmolb.2024.1446822
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/458291345
Introduction: Organoid models enable three-dimensional representation of cellular systems, providing flexible and accessible research tools, and can highlight key biomolecules. Such models of the intestinal epithelium can provide significant knowledge for the study of celiac disease and provide an additional context for the nature of markers observed from patient biopsy data.
Methods: Using LC-MS/MS, the proteomes of the crypt and enterocyte-like states of a mouse mini-gut organoid model were measured. The data were further compared with published biopsy data by comparing the changes induced by gluten challenge after a gluten-free diet.
Results and discussion: These analyses identified 4,850 protein groups and revealed how 400 putative biomarkers of dietary challenge were differentially expressed in the organoid model. In addition to the extensive changes within the differentiated cells, the data reiterated the disruption of the crypt-villus axis after gluten challenge. The mass spectrometry data are available via ProteomeXchange with the identifier PXD025690.
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The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The work was financially supported by Business Finland (Grant number 679/31/2015), Academy of Finland (no. 310011), the Finnish Cultural Foundation, Mary och Georg C. Ehrnrooths Stiftelse, Päivikki and Sakari Sohlberg Foundation and the State funding for University-level health research, Tampere University Hospital, Wellbeing Services County of Pirkanmaa. The Turku Bioscience Proteomics core is supported by Biocenter Finland. The work presented in this paper was conducted at Turku Bioscience Centre (University of Turku and Åbo Akademi University) and Tampere University. The proteomics analyses were carried out at the Turku Bioscience Proteomics core, which is supported by Biocenter Finland.
