Exploring the Interactions between Plant Proanthocyanidins and Thiabendazole : Insights from Isothermal Titration Calorimetry
: Sillanpää, Mimosa; Engström, Marica T.; Tähtinen, Petri; Green, Rebecca J.; Käpylä, Jarmo; Näreaho, Anu; Karonen, Maarit
Publisher: MPDI
: 2024
: Molecules
: Molecules (Basel, Switzerland)
: Molecules
: 3492
: 29
: 15
: 1420-3049
DOI: https://doi.org/10.3390/molecules29153492
: https://www.mdpi.com/1420-3049/29/15/3492
: https://research.utu.fi/converis/portal/detail/Publication/457541022
Anthelmintic resistance in gastrointestinal nematodes produces substantial challenges to agriculture, and new strategies for nematode control in livestock animals are called for. Natural compounds, including tannins, with proven anthelmintic activity could be a functional option as structurally diverse complementary compounds to be used alongside commercial anthelmintics. However, the dual use of two anthelmintic components requires an understanding of the pharmacological effects of the combination, while information concerning the interactions between plant-based polyphenols and commercial anthelmintics is scarce. We studied the direct interactions of proanthocyanidins (PAs, syn. condensed tannins) and a commercial anthelmintic thiabendazole, as a model substance of benzimidazoles, by isothermal titration calorimetry (ITC). Our results show evidence of a direct interaction of an exothermic nature with observed enthalpy changes ranging from 0 to -30 kJ/mol. The strength of the interaction between PAs and thiabendazole is mediated by structural characteristics of the PAs with the strongest positive correlation originating from the presence of galloyl groups and the increased degree of polymerization.
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This research was funded by the Research Council of Finland (LipidET project, decision 310549), Doctoral Programme in Physical and Chemical Sciences (PCS), Doctoral Programme in Exact Sciences (EXACTUS), University of Turku Joint Research Grant Fund, and The Finnish Concordia Fund.
