A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
HSV-1 and Cellular miRNAs in CSF-Derived Exosomes as Diagnostically Relevant Biomarkers for Neuroinflammation
Tekijät: Scheiber, Christian; Klein, Hans C.; Schneider, Julian M.; Schulz, Tanja; Bechter, Karl; Tumani, Hayrettin; Kapapa, Thomas; Flinkman, Dani ; Coffey, Eleanor; Ross, Duncan; Čistjakovs, Maksims; Nora-Krūkle, Zaiga; Bortolotti, Daria ; Rizzo, Roberta; Murovska, Modra; Schneider, E. M.
Kustantaja: MPDI
Julkaisuvuosi: 2024
Journal: Cells
Tietokannassa oleva lehden nimi: Cells
Lehden akronyymi: Cells
Artikkelin numero: 1208
Vuosikerta: 13
Numero: 14
eISSN: 2073-4409
DOI: https://doi.org/10.3390/cells13141208
Verkko-osoite: https://www.mdpi.com/2073-4409/13/14/1208
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/457340569
Virus-associated chronic inflammation may contribute to autoimmunity in a number of diseases. In the brain, autoimmune encephalitis appears related to fluctuating reactivation states of neurotropic viruses. In addition, viral miRNAs and proteins can be transmitted via exosomes, which constitute novel but highly relevant mediators of cellular communication. The current study questioned the role of HSV-1-encoded and host-derived miRNAs in cerebrospinal fluid (CSF)-derived exosomes, enriched from stress-induced neuroinflammatory diseases, mainly subarachnoid hemorrhage (SAH), psychiatric disorders (AF and SZ), and various other neuroinflammatory diseases. The results were compared with CSF exosomes from control donors devoid of any neuroinflammatory pathology. Serology proved positive, but variable immunity against herpesviruses in the majority of patients, except controls. Selective ultrastructural examinations identified distinct, herpesvirus-like particles in CSF-derived lymphocytes and monocytes. The likely release of extracellular vesicles and exosomes was most frequently observed from CSF monocytes. The exosomes released were structurally similar to highly purified stem-cell-derived exosomes. Exosomal RNA was quantified for HSV-1-derived miR-H2-3p, miR-H3-3p, miR-H4-3p, miR-H4-5p, miR-H6-3p, miR-H27 and host-derived miR-21-5p, miR-146a-5p, miR-155-5p, and miR-138-5p and correlated with the oxidative stress chemokine IL-8 and the axonal damage marker neurofilament light chain (NfL). Replication-associated miR-H27 correlated with neuronal damage marker NfL, and cell-derived miR-155-5p correlated with oxidative stress marker IL-8. Elevated miR-138-5p targeting HSV-1 latency-associated ICP0 inversely correlated with lower HSV-1 antibodies in CSF. In summary, miR-H27 and miR-155-5p may constitute neuroinflammatory markers for delineating frequent and fluctuating HSV-1 replication and NfL-related axonal damage in addition to the oxidative stress cytokine IL-8 in the brain. Tentatively, HSV-1 remains a relevant pathogen conditioning autoimmune processes and a psychiatric clinical phenotype.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
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This research was funded by the European Union’s Horizon 2020 research and innovation programs MOODSTRATIFICATION (grant agreement No. 754740, www.moodstratification.eu), and VirA (grant agreement No. 952376, www.vira-twinning.eu).
