A1 Refereed original research article in a scientific journal
Protocol to study early lung developmental branching in mouse embryos using explant culture
Authors: Talvi, Salli; Jokinen, Johanna; Rantakari, Pia; Heino, Jyrki
Publisher: Cell Press
Publication year: 2024
Journal: STAR protocols
Journal name in source: STAR Protocols
Article number: 103198
Volume: 5
Issue: 3
eISSN: 2666-1667
DOI: https://doi.org/10.1016/j.xpro.2024.103198
Web address : https://doi.org/10.1016/j.xpro.2024.103198
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/457268614
Mouse lung branching morphogenesis creates epithelial tree structures required for respiration. Here, we present a protocol for studying mouse lung developmental branching using lung explant cultures. We describe steps for isolating lungs with a video at embryonic day 12.5 (E12.5) and culturing as an explant for 2 days. We also detail procedures for microscopic imaging on days 0–2 and analysis of peripheral lung buds. This technique has the potential to investigate lung development in various conditions. For complete details on the use and execution of this protocol, please refer to Talvi et al.1
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Funding information in the publication:
This study has been financially supported by grants from the Academy of Finland (259769, J.H.), the Sigrid Juse´ lius Foundation (J.H.), the Cancer Foundation Finland (J.H.), the Finnish Foundation for Cardiovascular Research (J.H.), Research Council of Finland’s Flagship InFLAMES (337530 and 357910, J.H.), K. Albin Johanssons stiftelse (S.T.), Orion Research Foundation sr (S.T.), and the Ella and Georg Ehrnrooth Foundation (S.T.).
