Two new species of Zodariellum Andreeva & Tyshchenko, 1968 (Araneae: Zodariidae) are described on the basis of male holotypes collected in Central Asia: Z. doroshkini sp. n. from Gorno-Badakhshan in eastern Tajikistan, and Z. kattakum sp. n. from Surxondaryo in southeastern Uzbekistan. Additionally, Z. turanicum Zamani & Marusik, 2022 is recorded from Uzbekistan for the first time, and new distribution records are provided for Z. bactrianum (Kroneberg, 1875) and Z. mongolicum Marusik & Koponen, 2001. Detailed descriptions, figures, diagnoses, distribution maps and photographs of habitats of the studied species are provided.