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The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system and its pathways




TekijätAtosuo, Janne; Karhuvaara, Outi; Suominen, Eetu; Virtanen, Julia; Vilén, Liisa; Nuutila, Jari

KustantajaElsevier

Julkaisuvuosi2024

JournalJournal of Immunological Methods

Tietokannassa oleva lehden nimiJournal of immunological methods

Lehden akronyymiJ Immunol Methods

Artikkelin numero113709

Vuosikerta531

ISSN0022-1759

eISSN1872-7905

DOIhttps://doi.org/10.1016/j.jim.2024.113709

Verkko-osoitehttps://doi.org/10.1016/j.jim.2024.113709

Rinnakkaistallenteen osoitehttps://research.utu.fi/converis/portal/detail/Publication/456851264


Tiivistelmä
The complement system plays a crucial role in orchestrating the activation and regulation of inflammation within the human immune system. Three distinct activation pathways-classical, lectin, and alternative-converge to form the common lytic pathway, culminating in the formation of the membrane-attacking complex that disrupts the structure of pathogens. Dysregulated complement system activity can lead to tissue damage, autoimmune diseases, or immune deficiencies. In this study, the antimicrobial activity of human serum was investigated by using a bioluminescent microbe probe, Escherichia coli (pEGFPluxABCDEamp). This probe has previously been used to determine the antimicrobial activity of complement system and the polymorphonuclear neutrophils. In this study, blocking antibodies against key serum activators and components, including IgG, complement component 1q, factor B, and properdin, were utilized. The influence of body temperature and acute phase proteins, such as C reactive protein (CRP) and serum amyloid alpha (SAA), on the complement system was also examined. The study reveals the critical factors influencing complement system activity and pathway function. Alongside crucial factors like C1q and IgG, alternative pathway components factor B and properdin played pivotal roles. Results indicated that the alternative pathway accounted for approximately one third of the overall serum antimicrobial activity, and blocking this pathway disrupted the entire complement system. Contrary to expectations, elevated body temperature during inflammation did not enhance the antimicrobial activity of human serum. CRP demonstrated complement activation properties, but at higher physiological concentrations, it exhibited antagonistic tendencies, dampening the response. On the other hand, SAA enhanced the serum's activity. Overall, this study sheds a light on the critical factors affecting both complement system activity and pathway functionality, emphasizing the importance of a balanced immune response.

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Julkaisussa olevat rahoitustiedot
This work was funded by the Finnish Work Environment Fund (Finland) project 180090 and by the Parliament of Finland.


Last updated on 2025-27-01 at 19:35