A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
The gut microbiota is a major regulator of androgen metabolism in intestinal contents
Tekijät: Hannah Colldén, Andreas Landin, Ville Wallenius, Erik Elebring, Lars Fändriks, Maria E. Nilsson, Henrik Ryberg, Matti Poutanen, Klara Sjögren, Liesbeth Vandenput, Claes Ohlsson
Kustantaja: AMER PHYSIOLOGICAL SOC
Julkaisuvuosi: 2019
Journal: American Journal of Physiology : Endocrinology and Metabolism
Tietokannassa oleva lehden nimi: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
Lehden akronyymi: AM J PHYSIOL-ENDOC M
Vuosikerta: 317
Numero: 6
Aloitussivu: E1182
Lopetussivu: E1192
Sivujen määrä: 11
ISSN: 0193-1849
eISSN: 1522-1555
DOI: https://doi.org/10.1152/ajpendo.00338.2019
Verkko-osoite: https://journals.physiology.org/doi/full/10.1152/ajpendo.00338.2019
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/45388505
Androgens exert important effects both in androgen-responsive tissues and in the intestinal tract. To determine the impact of the gut microbiota (GM) on intestinal androgen metabolism, we measured unconjugated (free) and glucuronidated androgen levels in intestinal contents from the small intestine, with a low bacterial density, and from cecum and colon, with a high bacterial density. Using a specific, sensitive gas chromatography-tandem mass spectrometry method, we detected high levels of glucuronidated testosterone (T) and dihydrotestosterone (DHT) in small intestinal content of mice of both sexes, whereas in the distal intestine we observed remarkably high levels of free DHT, exceeding serum levels by >20-fold. Similarly, in young adult men high levels of unconjugated DHT, >70-fold higher than in serum, were detected in feces. In contrast to mice with a normal GM composition, germ-free mice had high levels of glucuronidated T and DHT, but very low free DHT levels, in the distal intestine. These findings demonstrate that the GM is involved in intestinal metabolism and deglucuronidation of DHT and T, resulting in extremely high free levels of the most potent androgen, DHT, in the colonic content of young and healthy mice and men.
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