A1 Refereed original research article in a scientific journal
GUN1 influences the accumulation of NEP-dependent transcripts and chloroplast protein import in Arabidopsis cotyledons upon perturbation of chloroplast protein homeostasis
Authors: Luca Tadini, Carlotta Peracchio, Andrea Trotta, Monica Colombo, Ilaria Mancini, Nicolaj Jeran, Alex Costa, Franco Faoro, Milena Marsoni, Candida Vannini, Eva‐Mari Aro, Paolo Pesaresi
Publisher: WILEY
Publication year: 2019
Journal: Plant Journal
Journal name in source: PLANT JOURNAL
Journal acronym: PLANT J
Volume: 101
Issue: 5
First page : 1198
Last page: 1220
Number of pages: 23
ISSN: 0960-7412
eISSN: 1365-313X
DOI: https://doi.org/10.1111/tpj.14585
Web address : https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14585
Correct chloroplast development and function require co-ordinated expression of chloroplast and nuclear genes. This is achieved through chloroplast signals that modulate nuclear gene expression in accordance with the chloroplast's needs. Genetic evidence indicates that GUN1, a chloroplast-localized pentatricopeptide repeat (PPR) protein with a C-terminal Small MutS-Related (SMR) domain, is involved in integrating multiple developmental and stress-related signals in both young seedlings and adult leaves. Recently, GUN1 was found to interact physically with factors involved in chloroplast protein homeostasis, and with enzymes of tetrapyrrole biosynthesis in adult leaves that function in various retrograde signalling pathways. Here we show that following perturbation of chloroplast protein homeostasis: (i) by growth in lincomycin-containing medium; or (ii) in mutants defective in either the FtsH protease complex (ftsh), plastid ribosome activity (prps21-1 and prpl11-1) or plastid protein import and folding (cphsc70-1), GUN1 influences NEP-dependent transcript accumulation during cotyledon greening and also intervenes in chloroplast protein import.
