A1 Refereed original research article in a scientific journal

Stacking up: a new approach for cell culture studies




AuthorsDiosangeles Soto Veliz, Hongbo Zhang, Martti Toivakka

PublisherROYAL SOC CHEMISTRY

Publication year2019

JournalBiomaterials Science

Journal name in sourceBIOMATERIALS SCIENCE

Journal acronymBIOMATER SCI-UK

Volume7

Issue8

First page 3249

Last page3257

Number of pages9

ISSN2047-4830

eISSN2047-4849

DOIhttps://doi.org/10.1039/c8bm01694a

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/42228522


Abstract
Traditional cell culture relies mostly on flat plastic surfaces, such as Petri dishes and multiwell plates. These commercial surfaces provide limited flexibility for experimental design. In contrast, cell biology increasingly demands surface customisation, functionalisation, and cell monitoring in order to obtain data that is relevant in vivo. The development of research areas such as microfluidics and electrochemical detection methods greatly promoted the customised design of cell culture platforms. However, the challenges for mass production and material limitations prevent their widespread usage and commercialisation. This article presents a new cell culture platform based on stacks of a transparent flexible printable substrate. The arrangement introduces multi-layered stacks for possible manipulation and access to the cells. The platform is highly compatible with current technologies, such as colorimetric imaging and fluorescence microscopy. In addition, it can potentially integrate, e.g., biomaterials, patterning, microfluidics, electrochemical detection and other techniques to influence, monitor, and assess cell behaviour in a multitude of different settings. More importantly, the platform is a low-cost alternative customisable through functional printing and coating technologies. The device shown in this manuscript represents a prototype for more sophisticated variations that will expand the relevance of in vitro studies in cell biology.

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