A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Quadruply-labeled serum albumin as a biodegradable nanosensor for simultaneous fluorescence imaging of intracellular pH values, oxygen and temperature
Tekijät: Xiao-ai Zhang, Wei Zhang, Qi Wang, Junli Wang, Guodong Ren, Xu-dong Wang
Kustantaja: SPRINGER WIEN
Julkaisuvuosi: 2019
Journal: Microchimica Acta
Tietokannassa oleva lehden nimi: MICROCHIMICA ACTA
Lehden akronyymi: MICROCHIM ACTA
Artikkelin numero: UNSP 584
Vuosikerta: 186
Numero: 8
Sivujen määrä: 11
ISSN: 0026-3672
eISSN: 1436-5073
DOI: https://doi.org/10.1007/s00604-019-3674-4
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/41795594
The construction of multiple fluorescent nanosensors for intracellular studies is a challenging task because spectral overlap of indicator probes can lead to cross-talk and mutual interference. This work describes biodegradable nanosensors that can simultaneously measure three intracellular parameters (temperature, pH and oxygen concentration). Bovine serum albumin (BSA) is selected as the scaffold to construct the triple nanosensor by covalent immobilization four fluorophores on BSA. The following luminophores were used: (a) fluorescein as a probe for pH values, (b) a platinum(II) porphyrin complex for oxygen; (c) a europium(III) clathrate complex for temperature, and (d) a rhodamine B as a reference dye. The nanoparticles have a size of 20nm and show excellent biocompatibility and good brightness. The nanosensors were used for ratiometric imaging of intracellular pH values, oxygen and temperature in HeLa cells. The triple nanosensor responds reversibly and this can be used for real-time tracing of these key parameters. Owing to their biodegradable feature, the use of this kind of triple nanosensor reduce the stress on cellular activities because less nanosensors can be used to gather the total information.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
