A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
3-Fluoro-2-mercuri-6-methylaniline Nucleotide as a High-Affinity Nucleobase-Specific Hybridization Probe
Tekijät: Aro-Heinilä A, Lönnberg T, Virta P
Kustantaja: American Chemical Society
Julkaisuvuosi: 2019
Journal: Bioconjugate Chemistry
Tietokannassa oleva lehden nimi: Bioconjugate Chemistry
Vuosikerta: 30
Numero: 8
Aloitussivu: 2183
Lopetussivu: 2190
Sivujen määrä: 8
ISSN: 1043-1802
eISSN: 1520-4812
DOI: https://doi.org/10.1021/acs.bioconjchem.9b00405
Verkko-osoite: https://doi.org/10.1021/acs.bioconjchem.9b00405
A 3-fluoro-6-methylaniline nucleoside was synthesized and incorporated
into an oligonucleotide, and its ability to form mercury-mediated base
pairs was studied. UV melting experiments revealed increased duplex
stability with thymine, guanine, and cytosine opposite to the probe and a
clear nucleobase-specific binding preference (T > G > C > A).
Moreover, the 3-fluoro group was utilized as a spin label that showed
distinct 19F NMR resonance shifts depending on the
complementary nucleobase, providing more detailed information on
Hg(II)-mediated base pairing.
