A1 Journal article – refereed

3-Fluoro-2-mercuri-6-methylaniline Nucleotide as a High-Affinity Nucleobase-Specific Hybridization Probe




List of Authors: Aro-Heinilä A, Lönnberg T, Virta P

Publisher: American Chemical Society

Publication year: 2019

Journal: Bioconjugate Chemistry

Journal name in source: Bioconjugate Chemistry

Volume number: 30

Issue number: 8

Number of pages: 8

ISSN: 1043-1802

eISSN: 1520-4812

DOI: http://dx.doi.org/10.1021/acs.bioconjchem.9b00405

URL: https://doi.org/10.1021/acs.bioconjchem.9b00405


Abstract

A 3-fluoro-6-methylaniline nucleoside was synthesized and incorporated
into an oligonucleotide, and its ability to form mercury-mediated base
pairs was studied. UV melting experiments revealed increased duplex
stability with thymine, guanine, and cytosine opposite to the probe and a
clear nucleobase-specific binding preference (T > G > C > A).
Moreover, the 3-fluoro group was utilized as a spin label that showed
distinct 19F NMR resonance shifts depending on the
complementary nucleobase, providing more detailed information on
Hg(II)-mediated base pairing.


Last updated on 2021-24-06 at 11:00