A1 Refereed original research article in a scientific journal
No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients
Authors: Lotta E Ivaska, Andreas Christensen, Matti Waris, Tuomo Puhakka, Tytti Vuorinen, Tobias Allander, Maria Söderlund-Venermo, Tuomas Jartti
Publisher: Oxford Academic Press
Publication year: 2019
Journal: Journal of Infectious Diseases
Volume: 220
Issue: 4
First page : 589
Last page: 593
Number of pages: 5
ISSN: 0022-1899
eISSN: 0022-1899
DOI: https://doi.org/10.1093/infdis/jiz166
Web address : 10.1093/infdis/jiz166
Human bocavirus 1 (HBoV1) can persist in nasopharynx and
tonsils. Using HBoV1 serology, reverse-transcription polymerase chain reaction (PCR) for detecting messenger RNA
(mRNA) and quantitative PCR for HBoV1 genome load count,
we studied to what extent the HBoV1 DNA loads in nasopharynx correlate with acute infection markers. Tonsillar tissue,
nasopharyngeal aspirate, and serum were obtained from 188
elective adeno-/tonsillectomy patients. Relatively high loads of
HBoV1 DNA were detected in the nasopharynx of 14 (7%) primarily asymptomatic subjects with negative mRNA and/or serodiagnostic results. Quantitative HBoV1 DNA PCR may have
lower specificity than HBoV1 mRNA detection for diagnosing
symptomatic infection.
