A1 Refereed original research article in a scientific journal
Anti-tumor activities of peptides corresponding to conserved complementary determining regions from different immunoglobulins
Authors: Figueiredo CR, Matsuo AL, Massaoka MH, Polonelli L, Travassos LR
Publication year: 2014
Journal: Peptides
Journal name in source: Peptides
Journal acronym: Peptides
Volume: 59
First page : 14
Last page: 9
Number of pages: 6
ISSN: 0196-9781
eISSN: 1873-5169
DOI: https://doi.org/10.1016/j.peptides.2014.06.007
Web address : https://reader.elsevier.com/reader/sd/pii/S0196978114001806?token=D6A01635AF17BCABA0D358FF2CC945CAAD60B7E1D244130CC67567419B73D8358A61DC0CA58CAF1F0A2776AC491ED3FD
Self-archived copy’s web address: https://reader.elsevier.com/reader/sd/pii/S0196978114001806?token=D6A01635AF17BCABA0D358FF2CC945CAAD60B7E1D244130CC67567419B73D8358A61DC0CA58CAF1F0A2776AC491ED3FD
Short synthetic peptides corresponding to sequences of complementarity-determining regions (CDRs) from different immunoglobulin families have been shown to induce antimicrobial, antiviral and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). Presently, we studied the in vitro and in vivo antitumor activity of synthetic peptides derived from conserved CDR sequences of different immunoglobulins against human tumor cell lines and murine B16F10-Nex2 melanoma aiming at the discovery of candidate molecules for cancer therapy. Four light- and heavy-chain CDR peptide sequences from different antibodies (C36-L1, HA9-H2, 1-H2 and Mg16-H2) showed cytotoxic activity against murine melanoma and a panel of human tumor cell lineages in vitro. Importantly, they also exerted anti-metastatic activity using a syngeneic melanoma model in mice. Other peptides (D07-H3, MN20v1, MS2-H3) were also protective against metastatic melanoma, without showing significant cytotoxicity against tumor cells in vitro. In this case, we suggest that these peptides may act as immune adjuvants in vivo. As observed, peptides induced nitric oxide production in bone-marrow macrophages showing that innate immune cells can also be modulated by these CDR peptides. The present screening supports the search in immunoglobulins of rather frequent CDR sequences that are endowed with specific antitumor properties and may be candidates to be developed as anti-cancer drugs.
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