A1 Refereed original research article in a scientific journal
Cumin Seed Oil Induces Oxidative Stress-Based Antifungal Activities on Fusarium graminearum
Authors: Yörük, Emre; Danışman, Zeynep; Pekmez, Murat; Yli-Mattila, Tapani
Publisher: MPDI
Publication year: 2024
Journal: Pathogens
Journal name in source: Pathogens
Article number: 395
Volume: 13
Issue: 5
ISSN: 2076-0817
eISSN: 2076-0817
DOI: https://doi.org/10.3390/pathogens13050395
Web address : https://www.mdpi.com/2076-0817/13/5/395
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/404641463
In this study, the antifungal activity of cumin seed oil (CSO) was tested on Fusarium graminearum. (i) Minimum inhibitory concentrations (MICs) and related concentrations (IC75, IC50, and IC25) were detected; (ii) toxicity was evaluated by a water-soluble tetrazolium salt-1 (WST-1) assay; (iii) genomic/epigenomic alterations were evaluated by the coupled restriction enzyme digestion-random amplification (CRED-RA) method; (iv) oxidative stress was investigated by CAT expression, catalase activity, and DCF-DA staining; (v) deoxynivalenol biosynthesis was evaluated by tri6 expression; (vi) and potential effects of CSO on wheat were tested by a water loss rate (WLR) assay. MIC, IC75, IC50 and IC25 values were detected at 0.5, 0.375, 0.25, and 0.125 mg mL−1. In WST-1 assays, significant decreases (p < 0.001) were detected. Genomic template stability (GTS) related to methylation differences ranged from 94.60% to 96.30%. Percentage polymorphism for HapII/MspI values were as 9.1%/15.8%. CAT (oxidative stress-related catalase) and tri6 (zinc finger motif transcription factor) gene expressions were recorded between 5.29 ± 0.74 and 0.46 ± 0.10 (p < 0.05). Increased catalase activity was detected (p < 0.05) by spectrophotometric assays. DCF-DA-stained (oxidative stressed) cells were increased in response to increased concentrations, and there were no significant changes in WLR values. It was concluded that CSO showed strong antifungal activity on F. graminearum via different physiological levels.
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