A1 Refereed original research article in a scientific journal

Peptide-oligonucleotide phosphorothioate conjugates with membrane translocation and nuclear localization properties



Authors: Antopolsky M, Azhayeva E, Tengvall U, Auriola S, Jaaskelainen I, Ronkko S, Honkakoski P, Urtti A, Lonnberg H, Azhayev A

Publisher: AMER CHEMICAL SOC

Publication year: 1999

Journal:: Bioconjugate Chemistry

Journal name in source: BIOCONJUGATE CHEMISTRY

Journal acronym: BIOCONJUGATE CHEM

Volume: 10

Issue: 4

First page : 598

Last page: 606

Number of pages: 9

ISSN: 1043-1802

DOI: https://doi.org/10.1021/bc980133y


Abstract
Eighteen peptide - oligonucleotide phosphorothioate conjugates were prepared in good yield and thoroughly characterized with electrospray ionization mass spectra. When applied to the living cells, conjugates exhibiting membrane translocation and nuclear localization properties displayed efficient intracellular penetration but failed to show any serious antisense effect. Studies on the intracellular distribution of the fluorescein-labeled conjugates revealed their trapping in endosomes.


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