A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Peptide-oligonucleotide phosphorothioate conjugates with membrane translocation and nuclear localization properties
Tekijät: Antopolsky M, Azhayeva E, Tengvall U, Auriola S, Jaaskelainen I, Ronkko S, Honkakoski P, Urtti A, Lonnberg H, Azhayev A
Kustantaja: AMER CHEMICAL SOC
Julkaisuvuosi: 1999
Lehti:: Bioconjugate Chemistry
Tietokannassa oleva lehden nimi: BIOCONJUGATE CHEMISTRY
Lehden akronyymi: BIOCONJUGATE CHEM
Vuosikerta: 10
Numero: 4
Aloitussivu: 598
Lopetussivu: 606
Sivujen määrä: 9
ISSN: 1043-1802
DOI: https://doi.org/10.1021/bc980133y
Tiivistelmä
Eighteen peptide - oligonucleotide phosphorothioate conjugates were prepared in good yield and thoroughly characterized with electrospray ionization mass spectra. When applied to the living cells, conjugates exhibiting membrane translocation and nuclear localization properties displayed efficient intracellular penetration but failed to show any serious antisense effect. Studies on the intracellular distribution of the fluorescein-labeled conjugates revealed their trapping in endosomes.
Eighteen peptide - oligonucleotide phosphorothioate conjugates were prepared in good yield and thoroughly characterized with electrospray ionization mass spectra. When applied to the living cells, conjugates exhibiting membrane translocation and nuclear localization properties displayed efficient intracellular penetration but failed to show any serious antisense effect. Studies on the intracellular distribution of the fluorescein-labeled conjugates revealed their trapping in endosomes.
Turun yliopiston Tutkimustietojärjestelmän portaali