Enhanced osteoblastic differentiation and bone formation in co-culture of human bone marrow mesenchymal stromal cells and peripheral blood mononuclear cells with exogenous VEGF - UTU Research Portal

A1 Refereed original research article in a scientific journal

Enhanced osteoblastic differentiation and bone formation in co-culture of human bone marrow mesenchymal stromal cells and peripheral blood mononuclear cells with exogenous VEGF

Authors: Joensuu K, Uusitalo L, Alm JJ, Aro HT, Hentunen TA, Heino TJ

Publisher: ELSEVIER MASSON

Publication year: 2015

Journal: Orthopaedics and Traumatology: Surgery and Research

Journal name in source: ORTHOPAEDICS & TRAUMATOLOGY-SURGERY & RESEARCH

Journal acronym: ORTHOP TRAUMATOL-SUR

Volume: 101

Issue: 3

First page : 381

Last page: 386

Number of pages: 6

ISSN: 1877-0568

DOI: https://doi.org/10.1016/j.otsr.2015.01.014(external)

Abstract

Conclusion: Our study demonstrates that the in vitro ALP activity and mineralization of human BM-MSCs is more efficient in the presence of PB-MNCs, and exogenously added VEGF further enhances the stimulatory effect. This indicates that PB-MNCs could be a potential cell source in development of co-culture systems for novel tissue engineering applications for enhanced bone healing. (C) 2015 Elsevier Masson SAS. All rights reserved.