A1 Refereed original research article in a scientific journal
Quantitative Proteomics Reveals the Dynamic Protein Landscape during Initiation of Human Th17 Cell Polarization
Authors: Tripathi SK, Välikangas T, Shetty A, Khan MM, Moulder R, Bhosale SD, Komsi E, Salo V, De Albuquerque RS, Rasool O, Galande S, Elo LL, Lahesmaa R
Publisher: CELL PRESS
Publication year: 2019
Journal: iScience
Journal name in source: ISCIENCE
Journal acronym: ISCIENCE
Volume: 11
First page : 334
Last page: 355
Number of pages: 44
ISSN: 2589-0042
eISSN: 2589-0042
DOI: https://doi.org/10.1016/j.isci.2018.12.020
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/39708259
Th17 cells contribute to the pathogenesis of inflammatory and autoimmune diseases and cancer. To reveal the Th17 cell-specific proteomic signature regulating Th17 cell differentiation and function in humans, we used a label-free mass spectrometry-based approach. Furthermore, a comprehensive analysis of the proteome and transcriptome of cells during human Th17 differentiation revealed a high degree of overlap between the datasets. However, when compared with corresponding published mouse data, we found very limited overlap between the proteins differentially regulated in response to Th17 differentiation. Validations were made for a panel of selected proteins with known and unknown functions. Finally, using RNA interference, we showed that SATB1 negatively regulates human Th17 cell differentiation. Overall, the current study illustrates a comprehensive picture of the global protein landscape during early human Th17 cell differentiation. Poor overlap with mouse data underlines the importance of human studies for translational research.
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