B1 Non-refereed article in a scientific journal
Base moiety selectivity in cleavage of short oligonucleotides by di- and trinuclear Zn(II) complexes of azacrown-derived ligands
Authors: Ketomaki K, Paivi-Poijarvi-Virta, Wang Q, Lonnberg H
Publication year: 2008
Journal:: Collection Symposium Series
Journal name in source: CHEMISTRY OF NUCLEIC ACID COMPONENTS
Journal acronym: COLL SYMPOS SERIES
Volume: 10
First page : 437
Last page: 438
Number of pages: 2
ISBN: 978-80-86241-29-6
Abstract
Cleavage of short oligoribonucleotides by a dinuclear Zn(2+) complex of 1,4-bis[(1,5,9-triazacyclododecan-3-yl)oxy methyl] benzene (L(1)) and trinuclear Zn(2+) complex of 1,3,5-tris[(1,5,9-triazacyclododecan-3-yl)oxymethyl] benzene (L(2)) has been studied. Zn(2+)L(1) at sufficiently low concentrations (< 0.1 mmol L(-1)) cleaves NpU and/or UpN bonds (N = G, C, A) much more readily than the other phosphodiester bonds. UpU sites, however, remain intact. Zn(2+)L(2), in turn, cleaves UpU bonds more readily than any other linkages, even more readily than NpU and UpN sites. This cleaving agent shows considerable cleaving activity also towards GpG sites.
Cleavage of short oligoribonucleotides by a dinuclear Zn(2+) complex of 1,4-bis[(1,5,9-triazacyclododecan-3-yl)oxy methyl] benzene (L(1)) and trinuclear Zn(2+) complex of 1,3,5-tris[(1,5,9-triazacyclododecan-3-yl)oxymethyl] benzene (L(2)) has been studied. Zn(2+)L(1) at sufficiently low concentrations (< 0.1 mmol L(-1)) cleaves NpU and/or UpN bonds (N = G, C, A) much more readily than the other phosphodiester bonds. UpU sites, however, remain intact. Zn(2+)L(2), in turn, cleaves UpU bonds more readily than any other linkages, even more readily than NpU and UpN sites. This cleaving agent shows considerable cleaving activity also towards GpG sites.
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