A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Solution-Phase Synthesis of Short Oligo-2'-deoxyribonucleotides by Using Clustered Nucleosides as a Soluble Support
Tekijät: Vyacheslav Kungurtsev, Jouni Laakkonen, Alejandro Gimenez Molina, Pasi Virta
Kustantaja: WILEY-V C H VERLAG GMBH
Julkaisuvuosi: 2013
Journal: European Journal of Organic Chemistry
Tietokannassa oleva lehden nimi: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Lehden akronyymi: EUR J ORG CHEM
Numero sarjassa: 29
Vuosikerta: 2013
Numero: 29
Aloitussivu: 6687
Lopetussivu: 6693
Sivujen määrä: 7
ISSN: 1434-193X
DOI: https://doi.org/10.1002/ejoc.201300864
Tiivistelmä
5-O-(4,4-Dimethoxytrityl)thymidine was attached to a pentaerythrityl-derived core, and the resulting tetravalent nucleoside cluster and the next dendritic generations served as a soluble support for the synthesis of short oligo-2-deoxyribonucleotides in solution. Couplings using a small excess (1.5 equiv. per 5-OH group) of the standard phosphoramidite building blocks proved efficient, and the products could be purified by quantitative precipitation from methanol. Ammonolysis released nearly homogeneous oligonucleotides (CCT, GCT, ACT, and AGCCT) in high yields.
5-O-(4,4-Dimethoxytrityl)thymidine was attached to a pentaerythrityl-derived core, and the resulting tetravalent nucleoside cluster and the next dendritic generations served as a soluble support for the synthesis of short oligo-2-deoxyribonucleotides in solution. Couplings using a small excess (1.5 equiv. per 5-OH group) of the standard phosphoramidite building blocks proved efficient, and the products could be purified by quantitative precipitation from methanol. Ammonolysis released nearly homogeneous oligonucleotides (CCT, GCT, ACT, and AGCCT) in high yields.
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