A1 Refereed original research article in a scientific journal
Solution-Phase Synthesis of Short Oligo-2'-deoxyribonucleotides by Using Clustered Nucleosides as a Soluble Support
Authors: Vyacheslav Kungurtsev, Jouni Laakkonen, Alejandro Gimenez Molina, Pasi Virta
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2013
Journal: European Journal of Organic Chemistry
Journal name in source: EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
Journal acronym: EUR J ORG CHEM
Number in series: 29
Volume: 2013
Issue: 29
First page : 6687
Last page: 6693
Number of pages: 7
ISSN: 1434-193X
DOI: https://doi.org/10.1002/ejoc.201300864
Abstract
5-O-(4,4-Dimethoxytrityl)thymidine was attached to a pentaerythrityl-derived core, and the resulting tetravalent nucleoside cluster and the next dendritic generations served as a soluble support for the synthesis of short oligo-2-deoxyribonucleotides in solution. Couplings using a small excess (1.5 equiv. per 5-OH group) of the standard phosphoramidite building blocks proved efficient, and the products could be purified by quantitative precipitation from methanol. Ammonolysis released nearly homogeneous oligonucleotides (CCT, GCT, ACT, and AGCCT) in high yields.
5-O-(4,4-Dimethoxytrityl)thymidine was attached to a pentaerythrityl-derived core, and the resulting tetravalent nucleoside cluster and the next dendritic generations served as a soluble support for the synthesis of short oligo-2-deoxyribonucleotides in solution. Couplings using a small excess (1.5 equiv. per 5-OH group) of the standard phosphoramidite building blocks proved efficient, and the products could be purified by quantitative precipitation from methanol. Ammonolysis released nearly homogeneous oligonucleotides (CCT, GCT, ACT, and AGCCT) in high yields.
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