Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria - UTU Research Portal

A1 Refereed original research article in a scientific journal

Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

Authors: Pu Xueyu, Wang Yuanyuan, Wang Xi, Sang Xiaoqing, Jiang Miaomiao, Qi DaWei, Zhao Xin, Chen Rong, Li Jianwei, Liu Xiang, Liu Zhidong, Yang Jian

Publisher: American Chemical Society

Publication year: 2024

Journal: Molecular Pharmaceutics

Journal name in source: Molecular pharmaceutics

Journal acronym: Mol Pharm

Volume: 21

Issue: 5

First page : 2238

Last page: 2249

ISSN: 1543-8384

eISSN: 1543-8392

DOI: https://doi.org/10.1021/acs.molpharmaceut.3c01001

Web address : https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.3c01001

Abstract

Tuberculosis (TB) is a chronic disease caused byMycobacterium tuberculosis (Mtb), which shows a long treatment cycle often leads to drug resistance, making treatment more difficult. Immunogens present in the pathogen's cell membrane can stimulate endogenous immune responses. Therefore, an effective lipid-based vaccine or drug delivery vehicle formulated from the pathogen's cell membrane can improve treatment outcomes. Herein, we extracted and characterized lipids fromMycobacterium smegmatis, and the extracts contained lipids belonging to numerous lipid classes and compounds typically found associated with mycobacteria. The extracted lipids were used to formulate biomimetic lipid reconstituted nanoparticles (LrNs) and LrNs-coated poly(lactic-co-glycolic acid) nanoparticles (PLGA-LrNs). Physiochemical characterization and results of morphology suggested that PLGA-LrNs exhibited enhanced stability compared with LrNs. And both of these two types of nanoparticles inhibited the growth of M. smegmatis. After loading different drugs, PLGA-LrNs containing berberine or coptisine strongly and synergistically prevented the growth of M. smegmatis. Altogether, the bacterial membrane lipids we extracted with antibacterial activity can be used as nanocarrier coating for synergistic antibacterial treatment of M. smegmatis─an alternative model of Mtb, which is expected as a novel therapeutic system for TB treatment.