Identification of stemness-related glycosylation changes in head and neck squamous cell carcinoma
: Routila Erica, Mahran Randa, Salminen Seppo, Irjala Heikki, Haapio Eeva, Kytö E, Ventelä Sami, Petterson Kim, Routila Johannes, Gidwani Kamlesh, Leivo Janne
Publisher: BioMed Central
: 2024
: BMC Cancer
: BMC cancer
: BMC Cancer
: 443
: 24
: 1471-2407
: 1471-2407
DOI: https://doi.org/10.1186/s12885-024-12161-5
: https://bmccancer.biomedcentral.com/articles/10.1186/s12885-024-12161-5
: https://research.utu.fi/converis/portal/detail/Publication/387645817
Background: Altered glycosylation is a hallmark of cancer associated with therapy resistance and tumor behavior. In this study, we investigated the glycosylation profile of stemness-related proteins OCT4, CIP2A, MET, and LIMA1 in HNSCC tumors.
Methods: Tumor, adjacent normal tissue, and blood samples of 25 patients were collected together with clinical details. After tissue processing, lectin-based glycovariant screens were performed.
Results: Strong correlation between glycosylation profiles of all four stemness-related proteins was observed in tumor tissue, whereas glycosylation in tumor tissue, adjacent normal tissue, and serum was differential.
Conclusions: A mannose- and galactose-rich glycosylation niche associated with stemness-related proteins was identified.
