A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Spectrally separated dual-label upconversion luminescence lateral flow assay for cancer-specific STn-glycosylation in CA125 and CA15-3
Tekijät: Ekman Miikka, Salminen Teppo, Raiko Kirsti, Soukka Tero, Gidwani Kamlesh, Martiskainen Iida
Kustantaja: Springer Nature
Julkaisuvuosi: 2024
Journal: Analytical and Bioanalytical Chemistry
Tietokannassa oleva lehden nimi: Analytical and bioanalytical chemistry
Lehden akronyymi: Anal Bioanal Chem
Vuosikerta: 416
Numero: 13
Aloitussivu: 3251
Lopetussivu: 3260
ISSN: 1618-2642
eISSN: 1618-2650
DOI: https://doi.org/10.1007/s00216-024-05275-z
Verkko-osoite: https://link.springer.com/article/10.1007/s00216-024-05275-z
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/387617668
Multiplexed lateral flow assays (LFAs) offer efficient on-site testing by simultaneously detecting multiple biomarkers from a single sample, reducing costs. In cancer diagnostics, where biomarkers can lack specificity, multiparameter detection provides more information at the point-of-care. Our research focuses on epithelial ovarian cancer (EOC), where STn-glycosylated forms of CA125 and CA15-3 antigens can better discriminate cancer from benign conditions. We have developed a dual-label LFA that detects both CA125-STn and CA15-3-STn within a single anti-STn antibody test line. This utilizes spectral separation of green (540 nm) and blue (450 nm) emitting erbium (NaYF4:Yb3+, Er3+)- and thulium (NaYF4: Yb3+, Tm3+)-doped upconverting nanoparticle (UCNP) reporters conjugated with antibodies against the protein epitopes in CA125 or CA15-3. This technology allows the simultaneous detection of different antigen variants from a single test line. The developed proof-of-concept dual-label LFA was able to distinguish between the ascites fluid samples from diagnosed ovarian cancer patients (n = 10) and liver cirrhosis ascites fluid samples (n = 3) used as a negative control. The analytical sensitivity of CA125-STn for the dual-label LFA was 1.8 U/ml in buffer and 3.6 U/ml in ascites fluid matrix. Here we demonstrate a novel approach of spectrally separated measurement of STn-glycosylated forms of two different cancer-associated protein biomarkers by using UCNP reporter technology.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
