A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Interactions of Galloylated Polyphenols with a Simple Gram-Negative Bacterial Membrane Lipid Model
Tekijät: Coones Ryan T., Karonen Maarit, Green Rebecca J., Frazier Richard
Kustantaja: MDPI
Kustannuspaikka: BASEL
Julkaisuvuosi: 2024
Journal: Membranes
Tietokannassa oleva lehden nimi: MEMBRANES
Lehden akronyymi: MEMBRANES-BASEL
Artikkelin numero: 47
Vuosikerta: 14
Numero: 2
Sivujen määrä: 13
eISSN: 2077-0375
DOI: https://doi.org/10.3390/membranes14020047
Verkko-osoite: https://doi.org/10.3390/membranes14020047
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/387350271
Differential scanning calorimetry (DSC) was used to explore the interactions of isolated polyphenolic compounds, including (-)-epigallocatechin gallate ((-)-EGCg), tellimagrandins I and II (Tel-I and Tel-II), and 1,2,3,4,6-penta-O-galloyl-d-glucose (PGG), with a model Gram-negative bacterial membrane with a view to investigating their antimicrobial properties. The model membranes comprised 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG), fabricated to mimic the domain formation observed in natural membranes, as well as ideally mixed lipid vesicles for the interaction with (-)-EGCg. Polyphenols induced changes in lipid mixing/de-mixing depending on the method of vesicle preparation, as was clearly evidenced by alterations in the lipid transition temperatures. There was a distinct affinity of the polyphenols for the DPPG lipid component, which was attributed to the electrostatic interactions between the polyphenolic galloyl moieties and the lipid headgroups. These interactions were found to operate through either the stabilization of the lipid headgroups by the polyphenols or the insertion of the polyphenols into the membrane itself. Structural attributes of the polyphenols, including the number of galloyl groups, the hydrophobicity quantified by partition coefficients (logP), and structural flexibility, exhibited a correlation with the temperature transitions observed in the DSC measurements. This study furthers our understanding of the intricate interplay between the structural features of polyphenolic compounds and their interactions with model bacterial membrane vesicles towards the exploitation of polyphenols as antimicrobials.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
