A1 Refereed original research article in a scientific journal

Exosomal miRNAs from neutrophils act as accurate biomarkers for gastric cancer diagnosis




AuthorsYu Dan, Zhang Jiahui, Wang Maoye, Ji Runbi, Qian Hui, Xu Wenrong, Zhang Hongbo, Gu Jianmei, Zhang Xu

PublisherElsevier B.V.

Publication year2024

JournalClinica Chimica Acta

Journal name in sourceClinica Chimica Acta

Volume554

eISSN1873-3492

DOIhttps://doi.org/10.1016/j.cca.2024.117773

Web address https://doi.org/10.1016/j.cca.2024.117773

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/387199996


Abstract

Background:

Gastric cancer (GC) is the third leading cause of cancer-related death worldwide. Sensitive and accurate biomarkers can greatly aid in early diagnosis and favorable prognosis. Neutrophils are the most abundant immune cells in human circulation and play a critical role in tumor progression. Neutrophil-derived exosomes (Neu-Exo) contain abundant bioactive molecules and are critically involved in disease progression.

Methods:

We proposed a Dynabeads-based (CD66b antibody-coupled) separation and detection system for Neu-Exo analysis. Dual antibody-assisted fluorescent Dynabeads was established to detect Neu-Exo abundance. MiRNA signature of Neu-Exo was identified by RNA sequencing. QRT-PCR and droplet digital PCR (ddPCR) were used for candidate miRNA detection and the potential of Neu-Exo miRNAs in the diagnosis of gastric cancer was evaluated.

Results:

Dual antibody-assisted fluorescent Dynabeads obtained a detection limit of 7.8 × 105 particles/mL of Neu-Exo and a recovery rate of 81 % under optimized conditions. ROC curve indicated that the abundance of CD66b+ Neu-Exo could well distinguish GC patients from healthy controls (HC) (AUC > 0.8). Additionally, miR-223-3p was found among the top differentially expressed miRNAs in Neu-Exo and presented superior diagnostic value in gastric cancer. Droplet digital PCR (ddPCR) significantly improved the diagnostic efficiency to differentiate GC patients from HC and benign gastric diseases (BGD) patients (AUC > 0.9).

Conclusion:

The Dynabeads-based separation and detection system, assisted with ddPCR analysis, provides a promising platform to enrich Neu-Exo and analyze miRNA profile for gastric cancer liquid biopsy.


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Last updated on 2024-26-11 at 11:59