Characterisation of experimental flowable composites containing fluoride-doped calcium phosphates as promising remineralising materials
: Alambiaga-Caravaca Adrián M., Chou Yu Fu, Moreno Daniel, Aparicio Conrado, López-Castellano Alicia, Feitosa Victor Pinheiro, Tezvergil-Mutluay Arzu, Sauro Salvatore
Publisher: Elsevier
: 2024
: Journal of Dentistry
: Journal of Dentistry
: 104906
: 143
: 0300-5712
: 1879-176X
DOI: https://doi.org/10.1016/j.jdent.2024.104906(external)
: https://doi.org/10.1016/j.jdent.2024.104906(external)
: https://research.utu.fi/converis/portal/detail/Publication/387109961(external)
Objective: Remineralising composites with antibacterial properties may seal the cavity and prevent secondary caries. This study aimed at developing experimental flowable composites containing different concentrations of fluoride-doped calcium phosphate fillers and evaluating their remineralising and antibacterial properties.
Methods: Experimental resin-based composites containing different concentrations (0-20 %) of fluoride-doped calcium phosphate fillers (VS10/VS20) were formulated. The release of calcium (Ca), phosphate (PO) and fluoride (F) ions was assessed for 30 days. Remineralisation properties were evaluated through ATR-FTIR and SEM/EDX after storage in simulated body fluid (SBF). The metabolic activity and viability of Streptococcus gordonii was also evaluated through ATP, CFU and live/dead confocal microscopy. The evaluation of specific monomer elution from the experimental composites was conducted using high-performance liquid chromatography (HPLC).
Results: The composites containing VS10 showed the highest release of Ca, those containing VS20 released more F over time (p < 0.05), while there was no significant difference in terms of PO ions release between the groups (p > 0.05). A quick 7-day mineral precipitation was observed in the tested composites containing VS10 or VS20 at 10 %; these materials also showed the greatest antibacterial activity (p < 0.05). Moreover, the tested composites containing VS10 presented the lowest elution of monomers (p < 0.05).
Conclusions: Innovative composites were developed with low monomers elution, evident antibacterial activity against S. gordonii and important remineralisation properties due to specific ions release.
Clinical significance: Novel composites containing fluoride-doped calcium phosphates may be promising to modulate bacteria growth, promote remineralisation and reduce the risk of cytotoxicity related to monomers' elution.