Fragmentation of thymidine induced by ultraviolet photoionization and thermal degradation

: Levola H, Kooser K, Rachlew E, Nommiste E, Kukk E

Publisher: ELSEVIER SCIENCE BV

: 2013

International Journal of Mass Spectrometry

: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

: INT J MASS SPECTROM

: 353

: 7

: 11

: 5

: 1387-3806

DOI: https://doi.org/10.1016/j.ijms.2013.08.008

Fragmentation of gas phase thymidine at different temperatures was studied with vacuum ultraviolet radiation and time-of-flight spectroscopy. Partial ion yield measurements were carried out in order to study the appearance energies of thymidine and its main fragments. The appearance energies of thymidine and its deoxyribose- and thymine-based fragments were determined to be 8.39, 8.77 and 8.81 eV, independently of temperature. The thermal decomposition of the sample was observed to start around 138-139 degrees C and it was explained by the breakage of the glycosidic bond, accompanied by hydrogen transfer from the deoxyribose ring to the thymine base. (C) 2013 Elsevier B.V. All rights reserved.

Appearance energy, Photodissociation, Photoionization, Thermal decomposition, Thymidine