A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Escherichia coli-expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen
Tekijät: Talha SM, Nemani SK, Salminen T, Kumar S, Swaminathan S, Soukka T, Pettersson K, Khanna N
Kustantaja: BIOMED CENTRAL LTD
Julkaisuvuosi: 2012
Journal: BMC Infectious Diseases
Tietokannassa oleva lehden nimi: BMC INFECTIOUS DISEASES
Lehden akronyymi: BMC INFECT DIS
Artikkelin numero: ARTN 325
Vuosikerta: 12
Sivujen määrä: 11
ISSN: 1471-2334
DOI: https://doi.org/10.1186/1471-2334-12-325
Tiivistelmä
This study has demonstrated the expression of r-gp160 variant in E. coli, by deletion of hydrophobic regions, and its purification in reasonable yields. This underscores the potential for cost saving in antigen production. Evaluation of this antigen in a double antigen sandwich two-step assay showed it to be a highly sensitive and specific HIV-1 diagnostic reagent. The amenability of this assay to the one-step format suggests its potential utility in developing a rapid point-of-care HIV-1 diagnostic test.
This study has demonstrated the expression of r-gp160 variant in E. coli, by deletion of hydrophobic regions, and its purification in reasonable yields. This underscores the potential for cost saving in antigen production. Evaluation of this antigen in a double antigen sandwich two-step assay showed it to be a highly sensitive and specific HIV-1 diagnostic reagent. The amenability of this assay to the one-step format suggests its potential utility in developing a rapid point-of-care HIV-1 diagnostic test.
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