A1 Refereed original research article in a scientific journal
Escherichia coli-expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen
Authors: Talha SM, Nemani SK, Salminen T, Kumar S, Swaminathan S, Soukka T, Pettersson K, Khanna N
Publisher: BIOMED CENTRAL LTD
Publication year: 2012
Journal: BMC Infectious Diseases
Journal name in source: BMC INFECTIOUS DISEASES
Journal acronym: BMC INFECT DIS
Article number: ARTN 325
Volume: 12
Number of pages: 11
ISSN: 1471-2334
DOI: https://doi.org/10.1186/1471-2334-12-325
Abstract
This study has demonstrated the expression of r-gp160 variant in E. coli, by deletion of hydrophobic regions, and its purification in reasonable yields. This underscores the potential for cost saving in antigen production. Evaluation of this antigen in a double antigen sandwich two-step assay showed it to be a highly sensitive and specific HIV-1 diagnostic reagent. The amenability of this assay to the one-step format suggests its potential utility in developing a rapid point-of-care HIV-1 diagnostic test.
This study has demonstrated the expression of r-gp160 variant in E. coli, by deletion of hydrophobic regions, and its purification in reasonable yields. This underscores the potential for cost saving in antigen production. Evaluation of this antigen in a double antigen sandwich two-step assay showed it to be a highly sensitive and specific HIV-1 diagnostic reagent. The amenability of this assay to the one-step format suggests its potential utility in developing a rapid point-of-care HIV-1 diagnostic test.
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