Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
: Luque-Uría Álvaro, Peltomaa Riikka, Navarro-Duro Marina, Fikacek Sabrina, Head Trajen, Deo Sapna, Daunert Sylvia, Benito-Peña Elena, Moreno-Bond María C.
Publisher: WILEY-V C H VERLAG GMBH
: WEINHEIM
: 2022
: Analysis & Sensing
: ANALYSIS & SENSING
: ANAL SENS
: e202100070
: 2
: 4
: 8
: 2629-2742
DOI: https://doi.org/10.1002/anse.202100070
: https://doi.org/10.1002/anse.202100070
: https://research.utu.fi/converis/portal/detail/Publication/381285229
The development of two different immunoassays for the determination of fumonisin B-1 in wheat samples is reported. A previously described mimopeptide for fumonisin B-1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2-GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61ngmL(-1) and a dynamic range from 1.9 to 95ngmL(-1), was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.
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