A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Birch inner bark procyanidins can be resolved with enhanced sensitivity by hydrophilic interaction HPLC-MS
Tekijät: Karonen M, Liimatainen J, Sinkkonen J
Kustantaja: WILEY PERIODICALS, INC
Julkaisuvuosi: 2011
Journal: Journal of Separation Science
Tietokannassa oleva lehden nimi: JOURNAL OF SEPARATION SCIENCE
Lehden akronyymi: J SEP SCI
Numero sarjassa: 22
Vuosikerta: 34
Numero: 22
Aloitussivu: 3158
Lopetussivu: 3165
Sivujen määrä: 8
ISSN: 1615-9306
DOI: https://doi.org/10.1002/jssc.201100569
Tiivistelmä
A complex mixture of procyanidin aglycones was isolated by Sephadex LH-20 column chromatography from the silver birch inner bark, which is a polyphenol-rich source of natural antioxidants. Procyanidins were studied by using hydrophilic interaction liquid chromatography combined with high-resolution ESI-TOF-MS. A good chromatographic separation was achieved and procyanidins eluted according to their increasing degree of polymerization. Individual procyanidins were detected from dimers up to the degree of polymerization of 22 by their negative-ion mass spectra. The results showed that hydrophilic interaction liquid chromatography can be successfully applied for the analysis of high-molecular-weight procyanidins with enhanced sensitivity in electrospray mass spectrometry.
A complex mixture of procyanidin aglycones was isolated by Sephadex LH-20 column chromatography from the silver birch inner bark, which is a polyphenol-rich source of natural antioxidants. Procyanidins were studied by using hydrophilic interaction liquid chromatography combined with high-resolution ESI-TOF-MS. A good chromatographic separation was achieved and procyanidins eluted according to their increasing degree of polymerization. Individual procyanidins were detected from dimers up to the degree of polymerization of 22 by their negative-ion mass spectra. The results showed that hydrophilic interaction liquid chromatography can be successfully applied for the analysis of high-molecular-weight procyanidins with enhanced sensitivity in electrospray mass spectrometry.
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