A1 Refereed original research article in a scientific journal
Birch inner bark procyanidins can be resolved with enhanced sensitivity by hydrophilic interaction HPLC-MS
Authors: Karonen M, Liimatainen J, Sinkkonen J
Publisher: WILEY PERIODICALS, INC
Publication year: 2011
Journal: Journal of Separation Science
Journal name in source: JOURNAL OF SEPARATION SCIENCE
Journal acronym: J SEP SCI
Number in series: 22
Volume: 34
Issue: 22
First page : 3158
Last page: 3165
Number of pages: 8
ISSN: 1615-9306
DOI: https://doi.org/10.1002/jssc.201100569
Abstract
A complex mixture of procyanidin aglycones was isolated by Sephadex LH-20 column chromatography from the silver birch inner bark, which is a polyphenol-rich source of natural antioxidants. Procyanidins were studied by using hydrophilic interaction liquid chromatography combined with high-resolution ESI-TOF-MS. A good chromatographic separation was achieved and procyanidins eluted according to their increasing degree of polymerization. Individual procyanidins were detected from dimers up to the degree of polymerization of 22 by their negative-ion mass spectra. The results showed that hydrophilic interaction liquid chromatography can be successfully applied for the analysis of high-molecular-weight procyanidins with enhanced sensitivity in electrospray mass spectrometry.
A complex mixture of procyanidin aglycones was isolated by Sephadex LH-20 column chromatography from the silver birch inner bark, which is a polyphenol-rich source of natural antioxidants. Procyanidins were studied by using hydrophilic interaction liquid chromatography combined with high-resolution ESI-TOF-MS. A good chromatographic separation was achieved and procyanidins eluted according to their increasing degree of polymerization. Individual procyanidins were detected from dimers up to the degree of polymerization of 22 by their negative-ion mass spectra. The results showed that hydrophilic interaction liquid chromatography can be successfully applied for the analysis of high-molecular-weight procyanidins with enhanced sensitivity in electrospray mass spectrometry.
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