A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Single nucleotide polymorphism (SNP) discovery in duplicated genomes: intron-primed exon-crossing (IPEC) as a strategy for avoiding amplification of duplicated loci in Atlantic salmon (Salmo salar) and other salmonid fishes
Tekijät: Ryynanen HJ, Primmer CR
Kustantaja: BIOMED CENTRAL LTD
Julkaisuvuosi: 2006
Lehti:: BMC Genomics
Tietokannassa oleva lehden nimi: BMC GENOMICS
Lehden akronyymi: BMC GENOMICS
Artikkelin numero: ARTN 192
Vuosikerta: 7
Sivujen määrä: 11
ISSN: 1471-2164
DOI: https://doi.org/10.1186/1471-2164-7-192
Tiivistelmä
Conclusion: Sequencing success was significantly higher with the new IPEC primers; thus the total number of loci to screen in order to identify one potential polymorphic site was six times less with this new strategy. Given that duplication may hamper SNP discovery in some species, the IPEC method reported here is an alternative way of identifying novel polymorphisms in such cases.
Conclusion: Sequencing success was significantly higher with the new IPEC primers; thus the total number of loci to screen in order to identify one potential polymorphic site was six times less with this new strategy. Given that duplication may hamper SNP discovery in some species, the IPEC method reported here is an alternative way of identifying novel polymorphisms in such cases.
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