A1 Refereed original research article in a scientific journal
Single nucleotide polymorphism (SNP) discovery in duplicated genomes: intron-primed exon-crossing (IPEC) as a strategy for avoiding amplification of duplicated loci in Atlantic salmon (Salmo salar) and other salmonid fishes
Authors: Ryynanen HJ, Primmer CR
Publisher: BIOMED CENTRAL LTD
Publication year: 2006
Journal:: BMC Genomics
Journal name in source: BMC GENOMICS
Journal acronym: BMC GENOMICS
Article number: ARTN 192
Volume: 7
Number of pages: 11
ISSN: 1471-2164
DOI: https://doi.org/10.1186/1471-2164-7-192
Abstract
Conclusion: Sequencing success was significantly higher with the new IPEC primers; thus the total number of loci to screen in order to identify one potential polymorphic site was six times less with this new strategy. Given that duplication may hamper SNP discovery in some species, the IPEC method reported here is an alternative way of identifying novel polymorphisms in such cases.
Conclusion: Sequencing success was significantly higher with the new IPEC primers; thus the total number of loci to screen in order to identify one potential polymorphic site was six times less with this new strategy. Given that duplication may hamper SNP discovery in some species, the IPEC method reported here is an alternative way of identifying novel polymorphisms in such cases.
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