A1 Journal article – refereed

Optimized native gel systems for separation of thylakoid protein complexes: novel super- and mega-complexes




List of Authors: Jarvi S, Suorsa M, Paakkarinen V, Aro EM

Publisher: PORTLAND PRESS LTD

Publication year: 2011

Journal: Biochemical Journal

Journal name in source: BIOCHEMICAL JOURNAL

Journal acronym: BIOCHEM J

Number in series: 2

Volume number: 439

Issue number: 2

Number of pages: 8

ISSN: 0264-6021

DOI: http://dx.doi.org/10.1042/BJ20102155


Abstract
Gel-based analysis of thylakoid membrane protein complexes represents a valuable tool to monitor the dynamics of the photosynthetic machinery. Native-PAGE preserves the components and often also the conformation of the protein complexes, thus enabling the analysis of their subunit composition. Nevertheless, the literature and practical experimentation in the field sometimes raise confusion owing to a great variety of native-PAGE and thylakoid-solubilization systems. In the present paper, we describe optimized methods for separation of higher plant thylakoid membrane protein complexes by native-PAGE addressing particularly: (i.) the use of detergent; (ii) the use of solubilization buffer; and (iii) the gel electrophoresis method. Special attention is paid to separation of high-molecular-mass thylakoid membrane super- and mega-complexes from Arabidopsis thaliana leaves. Several novel super- and mega-complexes including PS (photosystem) I, PSII and LHCs (light-harvesting complexes) in various combinations are reported.


Last updated on 2021-24-06 at 08:12