A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Optimized native gel systems for separation of thylakoid protein complexes: novel super- and mega-complexes
Tekijät: Jarvi S, Suorsa M, Paakkarinen V, Aro EM
Kustantaja: PORTLAND PRESS LTD
Julkaisuvuosi: 2011
Journal: Biochemical Journal
Tietokannassa oleva lehden nimi: BIOCHEMICAL JOURNAL
Lehden akronyymi: BIOCHEM J
Numero sarjassa: 2
Vuosikerta: 439
Numero: 2
Aloitussivu: 207
Lopetussivu: 214
Sivujen määrä: 8
ISSN: 0264-6021
DOI: https://doi.org/10.1042/BJ20102155
Tiivistelmä
Gel-based analysis of thylakoid membrane protein complexes represents a valuable tool to monitor the dynamics of the photosynthetic machinery. Native-PAGE preserves the components and often also the conformation of the protein complexes, thus enabling the analysis of their subunit composition. Nevertheless, the literature and practical experimentation in the field sometimes raise confusion owing to a great variety of native-PAGE and thylakoid-solubilization systems. In the present paper, we describe optimized methods for separation of higher plant thylakoid membrane protein complexes by native-PAGE addressing particularly: (i.) the use of detergent; (ii) the use of solubilization buffer; and (iii) the gel electrophoresis method. Special attention is paid to separation of high-molecular-mass thylakoid membrane super- and mega-complexes from Arabidopsis thaliana leaves. Several novel super- and mega-complexes including PS (photosystem) I, PSII and LHCs (light-harvesting complexes) in various combinations are reported.
Gel-based analysis of thylakoid membrane protein complexes represents a valuable tool to monitor the dynamics of the photosynthetic machinery. Native-PAGE preserves the components and often also the conformation of the protein complexes, thus enabling the analysis of their subunit composition. Nevertheless, the literature and practical experimentation in the field sometimes raise confusion owing to a great variety of native-PAGE and thylakoid-solubilization systems. In the present paper, we describe optimized methods for separation of higher plant thylakoid membrane protein complexes by native-PAGE addressing particularly: (i.) the use of detergent; (ii) the use of solubilization buffer; and (iii) the gel electrophoresis method. Special attention is paid to separation of high-molecular-mass thylakoid membrane super- and mega-complexes from Arabidopsis thaliana leaves. Several novel super- and mega-complexes including PS (photosystem) I, PSII and LHCs (light-harvesting complexes) in various combinations are reported.
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