A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Sixty-two new microsatellite markers for an endemic frog Indirana beddomii from the Western Ghats biodiversity hotspot
Tekijät: Nair A, Kumar KS, George S, Gopalan SV, Li MH, Leder EH, Merila J
Kustantaja: SPRINGER
Julkaisuvuosi: 2011
Journal: Conservation Genetics Resources
Tietokannassa oleva lehden nimi: CONSERVATION GENETICS RESOURCES
Lehden akronyymi: CONSERV GENET RESOUR
Numero sarjassa: 1
Vuosikerta: 3
Numero: 1
Aloitussivu: 167
Lopetussivu: 171
Sivujen määrä: 5
ISSN: 1877-7252
DOI: https://doi.org/10.1007/s12686-010-9315-1
Tiivistelmä
Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2-20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs.
Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2-20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs.
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