A1 Refereed original research article in a scientific journal
Sixty-two new microsatellite markers for an endemic frog Indirana beddomii from the Western Ghats biodiversity hotspot
Authors: Nair A, Kumar KS, George S, Gopalan SV, Li MH, Leder EH, Merila J
Publisher: SPRINGER
Publication year: 2011
Journal: Conservation Genetics Resources
Journal name in source: CONSERVATION GENETICS RESOURCES
Journal acronym: CONSERV GENET RESOUR
Number in series: 1
Volume: 3
Issue: 1
First page : 167
Last page: 171
Number of pages: 5
ISSN: 1877-7252
DOI: https://doi.org/10.1007/s12686-010-9315-1
Abstract
Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2-20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs.
Frogs in the genus Indirana are endemic to Western Ghats biodiversity hotspot. The species are poorly studied and in many cases threatened or endangered. Here we describe primers and polymerase chain reactions for 62 microsatellite loci for Indirana beddomii, one of the commonest frogs in the genus. Fifty-six of the primers were polymorphic on sample of 23 individuals from a single sampling site (Ponmudi, Kerala) with an average 9.11 alleles per locus (range = 2-20). The average observed and expected heterozygosities were 0.64 and 0.71, respectively. The loci should be useful in conservation genetic studies of Indirana frogs.
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