A1 Refereed original research article in a scientific journal
Complete genomic sequence of the lytic bacteriophage phi YeO3-12 of Yersinia enterocolitica serotype O : 3
Authors: Pajunen MI, Kiljunen SJ, Soderholm MEL, Skurnik M
Publisher: AMER SOC MICROBIOLOGY
Publication year: 2001
Journal:: Journal of Bacteriology
Journal name in source: JOURNAL OF BACTERIOLOGY
Journal acronym: J BACTERIOL
Volume: 183
Issue: 6
First page : 1928
Last page: 1937
Number of pages: 10
ISSN: 0021-9193
DOI: https://doi.org/10.1128/JB.183.6.1928-1937.2001
Web address : http://jb.asm.org/content/183/6/1928.full
Abstract
phi YeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3, The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the phi YeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted phi YeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, phi YeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonnei D(2)371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik J. Bacteriol. 182:5114-5120, 2000). These findings indicate that phi YeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.
phi YeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3, The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the phi YeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted phi YeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, phi YeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonnei D(2)371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik J. Bacteriol. 182:5114-5120, 2000). These findings indicate that phi YeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.
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